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h3k9me3 w45a biosensor addgene  (Addgene inc)


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    Structured Review

    Addgene inc h3k9me3 w45a biosensor addgene
    H3k9me3 W45a Biosensor Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h3k9me3+w45a+biosensor+addgene/W45A+mutant+of+H3K9me3+biosensor+(Plasmid+%23120808)/10__1016_slash_j__isci__2025__113743-299-167-170
    Average 93 stars, based on 1 article reviews
    h3k9me3 w45a biosensor addgene - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Recombinant:

    Article Title: Robust calibration and quantification of FRET signals using multiplexed biosensor barcoding
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins EGF Sigma-Aldrich Cat#E9644 Tranylcypromine (TCP) MilliporeSigma Cat#616431 Critical commercial assays Gateway BP Clonase II Invitrogen Cat#11789020 Gateway LR Clonase II Invitrogen Cat#11791020 GenJet In Vitro DNA Transfection Reagent SignaGen Laboratories Cat#SL100489 Lenti-X Concentrator Takara Bio Cat#631231 Experimental models: Cell lines Human: HEK293T ATCC CRL-3216 Human: HeLa ATCC CCL-2 Human: SiHa ATCC HTB-35 Oligonucleotides Forward primer to add attB1 for EKAR1, CytoFAK, FRET-ON: GGGGACAAGTTTGTACAAAAAAGCAGGCTTCACCATGGTGA GCAAGGGCGAGGAGC This paper N/A Reverse primer to add attB2 and stop codon for EKAR1, CytoFAK, FRET-ON: GGGGACCACTTTGTACAAGAAAGCTGGGTCTCATTTGTACAA TTCATTCATACCCTCGG This paper N/A Jo urn al Pr e-p roo f 16 Forward primer to add attB1 for H3K9me3, H3K9me3 (W45A), FRET-OFF: GGGGACAAGTTTGTACAAAAAAGCAGGCTGCCACCATGTCT AAAGGTGAAGAATTATTCACTGGTGTTGTCCC This paper N/A Reverse primer to add attB2 and stop codon for H3K9me3, H3K9me3 (W45A), FRET-OFF: GGGGACCACTTTGTACAAGAAAGCTGGGTCTAGGCGGCGG TCACGAACTCC This paper N/A Forward primer for CytoFAK Y349F mutation: GGGTTCTGAGACCGACGACTTCGCTGAGATCATCGACGAGG This paper N/A Reverse primer for CytoFAK Y349F mutation: CCTCGTCGATGATCTCAGCGAAGTCGTCGGTCTCAGAACCC This paper N/A Recombinant DNA EKAR Addgene Cat#18679 CytoFAK Addgene Cat#78300 H3K9me3 biosensor Addgene Cat#120802 H3K9me3 (W45A) biosensor Addgene Cat#120808 FRET-ON This paper N/A FRET-OFF This paper N/A EKAR1 This paper N/A pDONR221 vector Invitrogen Cat#12536017 pLenti-CMV-Neo destination vector Addgene Cat#17392 pMD2.G Addgene Cat#12259 psPAX2 Addgene Cat#12260 Jo urn al Pr e-p roo f 17 Software and algorithms Zen Microscopy Software Zeiss https://www.zeiss.com/micros copy/us/home.html ImageJ/Fiji Schindelin et al., 201262; Schneider et al., 201263 https://imagej.net/software/fiji/ Jo urn al Pr e-p roo f 18

    In Vitro:

    Article Title: Robust calibration and quantification of FRET signals using multiplexed biosensor barcoding
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins EGF Sigma-Aldrich Cat#E9644 Tranylcypromine (TCP) MilliporeSigma Cat#616431 Critical commercial assays Gateway BP Clonase II Invitrogen Cat#11789020 Gateway LR Clonase II Invitrogen Cat#11791020 GenJet In Vitro DNA Transfection Reagent SignaGen Laboratories Cat#SL100489 Lenti-X Concentrator Takara Bio Cat#631231 Experimental models: Cell lines Human: HEK293T ATCC CRL-3216 Human: HeLa ATCC CCL-2 Human: SiHa ATCC HTB-35 Oligonucleotides Forward primer to add attB1 for EKAR1, CytoFAK, FRET-ON: GGGGACAAGTTTGTACAAAAAAGCAGGCTTCACCATGGTGA GCAAGGGCGAGGAGC This paper N/A Reverse primer to add attB2 and stop codon for EKAR1, CytoFAK, FRET-ON: GGGGACCACTTTGTACAAGAAAGCTGGGTCTCATTTGTACAA TTCATTCATACCCTCGG This paper N/A Jo urn al Pr e-p roo f 16 Forward primer to add attB1 for H3K9me3, H3K9me3 (W45A), FRET-OFF: GGGGACAAGTTTGTACAAAAAAGCAGGCTGCCACCATGTCT AAAGGTGAAGAATTATTCACTGGTGTTGTCCC This paper N/A Reverse primer to add attB2 and stop codon for H3K9me3, H3K9me3 (W45A), FRET-OFF: GGGGACCACTTTGTACAAGAAAGCTGGGTCTAGGCGGCGG TCACGAACTCC This paper N/A Forward primer for CytoFAK Y349F mutation: GGGTTCTGAGACCGACGACTTCGCTGAGATCATCGACGAGG This paper N/A Reverse primer for CytoFAK Y349F mutation: CCTCGTCGATGATCTCAGCGAAGTCGTCGGTCTCAGAACCC This paper N/A Recombinant DNA EKAR Addgene Cat#18679 CytoFAK Addgene Cat#78300 H3K9me3 biosensor Addgene Cat#120802 H3K9me3 (W45A) biosensor Addgene Cat#120808 FRET-ON This paper N/A FRET-OFF This paper N/A EKAR1 This paper N/A pDONR221 vector Invitrogen Cat#12536017 pLenti-CMV-Neo destination vector Addgene Cat#17392 pMD2.G Addgene Cat#12259 psPAX2 Addgene Cat#12260 Jo urn al Pr e-p roo f 17 Software and algorithms Zen Microscopy Software Zeiss https://www.zeiss.com/micros copy/us/home.html ImageJ/Fiji Schindelin et al., 201262; Schneider et al., 201263 https://imagej.net/software/fiji/ Jo urn al Pr e-p roo f 18

    Transfection:

    Article Title: Robust calibration and quantification of FRET signals using multiplexed biosensor barcoding
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins EGF Sigma-Aldrich Cat#E9644 Tranylcypromine (TCP) MilliporeSigma Cat#616431 Critical commercial assays Gateway BP Clonase II Invitrogen Cat#11789020 Gateway LR Clonase II Invitrogen Cat#11791020 GenJet In Vitro DNA Transfection Reagent SignaGen Laboratories Cat#SL100489 Lenti-X Concentrator Takara Bio Cat#631231 Experimental models: Cell lines Human: HEK293T ATCC CRL-3216 Human: HeLa ATCC CCL-2 Human: SiHa ATCC HTB-35 Oligonucleotides Forward primer to add attB1 for EKAR1, CytoFAK, FRET-ON: GGGGACAAGTTTGTACAAAAAAGCAGGCTTCACCATGGTGA GCAAGGGCGAGGAGC This paper N/A Reverse primer to add attB2 and stop codon for EKAR1, CytoFAK, FRET-ON: GGGGACCACTTTGTACAAGAAAGCTGGGTCTCATTTGTACAA TTCATTCATACCCTCGG This paper N/A Jo urn al Pr e-p roo f 16 Forward primer to add attB1 for H3K9me3, H3K9me3 (W45A), FRET-OFF: GGGGACAAGTTTGTACAAAAAAGCAGGCTGCCACCATGTCT AAAGGTGAAGAATTATTCACTGGTGTTGTCCC This paper N/A Reverse primer to add attB2 and stop codon for H3K9me3, H3K9me3 (W45A), FRET-OFF: GGGGACCACTTTGTACAAGAAAGCTGGGTCTAGGCGGCGG TCACGAACTCC This paper N/A Forward primer for CytoFAK Y349F mutation: GGGTTCTGAGACCGACGACTTCGCTGAGATCATCGACGAGG This paper N/A Reverse primer for CytoFAK Y349F mutation: CCTCGTCGATGATCTCAGCGAAGTCGTCGGTCTCAGAACCC This paper N/A Recombinant DNA EKAR Addgene Cat#18679 CytoFAK Addgene Cat#78300 H3K9me3 biosensor Addgene Cat#120802 H3K9me3 (W45A) biosensor Addgene Cat#120808 FRET-ON This paper N/A FRET-OFF This paper N/A EKAR1 This paper N/A pDONR221 vector Invitrogen Cat#12536017 pLenti-CMV-Neo destination vector Addgene Cat#17392 pMD2.G Addgene Cat#12259 psPAX2 Addgene Cat#12260 Jo urn al Pr e-p roo f 17 Software and algorithms Zen Microscopy Software Zeiss https://www.zeiss.com/micros copy/us/home.html ImageJ/Fiji Schindelin et al., 201262; Schneider et al., 201263 https://imagej.net/software/fiji/ Jo urn al Pr e-p roo f 18

    Mutagenesis:

    Article Title: Robust calibration and quantification of FRET signals using multiplexed biosensor barcoding
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins EGF Sigma-Aldrich Cat#E9644 Tranylcypromine (TCP) MilliporeSigma Cat#616431 Critical commercial assays Gateway BP Clonase II Invitrogen Cat#11789020 Gateway LR Clonase II Invitrogen Cat#11791020 GenJet In Vitro DNA Transfection Reagent SignaGen Laboratories Cat#SL100489 Lenti-X Concentrator Takara Bio Cat#631231 Experimental models: Cell lines Human: HEK293T ATCC CRL-3216 Human: HeLa ATCC CCL-2 Human: SiHa ATCC HTB-35 Oligonucleotides Forward primer to add attB1 for EKAR1, CytoFAK, FRET-ON: GGGGACAAGTTTGTACAAAAAAGCAGGCTTCACCATGGTGA GCAAGGGCGAGGAGC This paper N/A Reverse primer to add attB2 and stop codon for EKAR1, CytoFAK, FRET-ON: GGGGACCACTTTGTACAAGAAAGCTGGGTCTCATTTGTACAA TTCATTCATACCCTCGG This paper N/A Jo urn al Pr e-p roo f 16 Forward primer to add attB1 for H3K9me3, H3K9me3 (W45A), FRET-OFF: GGGGACAAGTTTGTACAAAAAAGCAGGCTGCCACCATGTCT AAAGGTGAAGAATTATTCACTGGTGTTGTCCC This paper N/A Reverse primer to add attB2 and stop codon for H3K9me3, H3K9me3 (W45A), FRET-OFF: GGGGACCACTTTGTACAAGAAAGCTGGGTCTAGGCGGCGG TCACGAACTCC This paper N/A Forward primer for CytoFAK Y349F mutation: GGGTTCTGAGACCGACGACTTCGCTGAGATCATCGACGAGG This paper N/A Reverse primer for CytoFAK Y349F mutation: CCTCGTCGATGATCTCAGCGAAGTCGTCGGTCTCAGAACCC This paper N/A Recombinant DNA EKAR Addgene Cat#18679 CytoFAK Addgene Cat#78300 H3K9me3 biosensor Addgene Cat#120802 H3K9me3 (W45A) biosensor Addgene Cat#120808 FRET-ON This paper N/A FRET-OFF This paper N/A EKAR1 This paper N/A pDONR221 vector Invitrogen Cat#12536017 pLenti-CMV-Neo destination vector Addgene Cat#17392 pMD2.G Addgene Cat#12259 psPAX2 Addgene Cat#12260 Jo urn al Pr e-p roo f 17 Software and algorithms Zen Microscopy Software Zeiss https://www.zeiss.com/micros copy/us/home.html ImageJ/Fiji Schindelin et al., 201262; Schneider et al., 201263 https://imagej.net/software/fiji/ Jo urn al Pr e-p roo f 18

    Software:

    Article Title: Robust calibration and quantification of FRET signals using multiplexed biosensor barcoding
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins EGF Sigma-Aldrich Cat#E9644 Tranylcypromine (TCP) MilliporeSigma Cat#616431 Critical commercial assays Gateway BP Clonase II Invitrogen Cat#11789020 Gateway LR Clonase II Invitrogen Cat#11791020 GenJet In Vitro DNA Transfection Reagent SignaGen Laboratories Cat#SL100489 Lenti-X Concentrator Takara Bio Cat#631231 Experimental models: Cell lines Human: HEK293T ATCC CRL-3216 Human: HeLa ATCC CCL-2 Human: SiHa ATCC HTB-35 Oligonucleotides Forward primer to add attB1 for EKAR1, CytoFAK, FRET-ON: GGGGACAAGTTTGTACAAAAAAGCAGGCTTCACCATGGTGA GCAAGGGCGAGGAGC This paper N/A Reverse primer to add attB2 and stop codon for EKAR1, CytoFAK, FRET-ON: GGGGACCACTTTGTACAAGAAAGCTGGGTCTCATTTGTACAA TTCATTCATACCCTCGG This paper N/A Jo urn al Pr e-p roo f 16 Forward primer to add attB1 for H3K9me3, H3K9me3 (W45A), FRET-OFF: GGGGACAAGTTTGTACAAAAAAGCAGGCTGCCACCATGTCT AAAGGTGAAGAATTATTCACTGGTGTTGTCCC This paper N/A Reverse primer to add attB2 and stop codon for H3K9me3, H3K9me3 (W45A), FRET-OFF: GGGGACCACTTTGTACAAGAAAGCTGGGTCTAGGCGGCGG TCACGAACTCC This paper N/A Forward primer for CytoFAK Y349F mutation: GGGTTCTGAGACCGACGACTTCGCTGAGATCATCGACGAGG This paper N/A Reverse primer for CytoFAK Y349F mutation: CCTCGTCGATGATCTCAGCGAAGTCGTCGGTCTCAGAACCC This paper N/A Recombinant DNA EKAR Addgene Cat#18679 CytoFAK Addgene Cat#78300 H3K9me3 biosensor Addgene Cat#120802 H3K9me3 (W45A) biosensor Addgene Cat#120808 FRET-ON This paper N/A FRET-OFF This paper N/A EKAR1 This paper N/A pDONR221 vector Invitrogen Cat#12536017 pLenti-CMV-Neo destination vector Addgene Cat#17392 pMD2.G Addgene Cat#12259 psPAX2 Addgene Cat#12260 Jo urn al Pr e-p roo f 17 Software and algorithms Zen Microscopy Software Zeiss https://www.zeiss.com/micros copy/us/home.html ImageJ/Fiji Schindelin et al., 201262; Schneider et al., 201263 https://imagej.net/software/fiji/ Jo urn al Pr e-p roo f 18

    Microscopy:

    Article Title: Robust calibration and quantification of FRET signals using multiplexed biosensor barcoding
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins EGF Sigma-Aldrich Cat#E9644 Tranylcypromine (TCP) MilliporeSigma Cat#616431 Critical commercial assays Gateway BP Clonase II Invitrogen Cat#11789020 Gateway LR Clonase II Invitrogen Cat#11791020 GenJet In Vitro DNA Transfection Reagent SignaGen Laboratories Cat#SL100489 Lenti-X Concentrator Takara Bio Cat#631231 Experimental models: Cell lines Human: HEK293T ATCC CRL-3216 Human: HeLa ATCC CCL-2 Human: SiHa ATCC HTB-35 Oligonucleotides Forward primer to add attB1 for EKAR1, CytoFAK, FRET-ON: GGGGACAAGTTTGTACAAAAAAGCAGGCTTCACCATGGTGA GCAAGGGCGAGGAGC This paper N/A Reverse primer to add attB2 and stop codon for EKAR1, CytoFAK, FRET-ON: GGGGACCACTTTGTACAAGAAAGCTGGGTCTCATTTGTACAA TTCATTCATACCCTCGG This paper N/A Jo urn al Pr e-p roo f 16 Forward primer to add attB1 for H3K9me3, H3K9me3 (W45A), FRET-OFF: GGGGACAAGTTTGTACAAAAAAGCAGGCTGCCACCATGTCT AAAGGTGAAGAATTATTCACTGGTGTTGTCCC This paper N/A Reverse primer to add attB2 and stop codon for H3K9me3, H3K9me3 (W45A), FRET-OFF: GGGGACCACTTTGTACAAGAAAGCTGGGTCTAGGCGGCGG TCACGAACTCC This paper N/A Forward primer for CytoFAK Y349F mutation: GGGTTCTGAGACCGACGACTTCGCTGAGATCATCGACGAGG This paper N/A Reverse primer for CytoFAK Y349F mutation: CCTCGTCGATGATCTCAGCGAAGTCGTCGGTCTCAGAACCC This paper N/A Recombinant DNA EKAR Addgene Cat#18679 CytoFAK Addgene Cat#78300 H3K9me3 biosensor Addgene Cat#120802 H3K9me3 (W45A) biosensor Addgene Cat#120808 FRET-ON This paper N/A FRET-OFF This paper N/A EKAR1 This paper N/A pDONR221 vector Invitrogen Cat#12536017 pLenti-CMV-Neo destination vector Addgene Cat#17392 pMD2.G Addgene Cat#12259 psPAX2 Addgene Cat#12260 Jo urn al Pr e-p roo f 17 Software and algorithms Zen Microscopy Software Zeiss https://www.zeiss.com/micros copy/us/home.html ImageJ/Fiji Schindelin et al., 201262; Schneider et al., 201263 https://imagej.net/software/fiji/ Jo urn al Pr e-p roo f 18



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    Addgene inc h3k9me3 w45a biosensor addgene
    H3k9me3 W45a Biosensor Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h3k9me3+w45a+biosensor+addgene/W45A+mutant+of+H3K9me3+biosensor+(Plasmid+%23120808)/10__1016_slash_j__isci__2025__113743-299-167-170
    Average 93 stars, based on 1 article reviews
    h3k9me3 w45a biosensor addgene - by Bioz Stars, 2026-10
    93/100 stars
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